T-DNA–mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants (2024)

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Abstract Access options Additional access options: Similar content being viewed by others Regulation of gene editing using T-DNA concatenation Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana Distinct mechanisms for genomic attachment of the 5′ and 3′ ends of Agrobacterium T-DNA in plants References Acknowledgements Author information Authors and Affiliations Corresponding authors Supplementary information Supplementary Text and Figures Rights and permissions About this article Cite this article This article is cited by Foreign DNA detection in genome-edited potatoes by high-throughput sequencing Widespread occurrence of natural genetic transformation of plants by Agrobacterium Extensive transcriptomic and epigenomic remodelling occurs during Arabidopsis thaliana germination Efficient Construction of Large Genomic Deletion in Agrobacterium tumefaciens by Combination of Cre/loxP System and Triple Recombineering Biolistic transformation of wheat: increased production of plants with simple insertions and heritable transgene expression
  • Brief Communication
  • Published:
  • Bekir Ülker1nAff4,
  • Yong Li2nAff4,
  • Mario G Rosso2nAff4,
  • Elke Logemann1,
  • Imre E Somssich1 &
  • …
  • Bernd Weisshaar2,3

Nature Biotechnology volume26,pages 1015–1017 (2008)Cite this article

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Abstract

Besides the well-documented integration of DNA flanked by the transfer DNA borders, occasional insertion of fragments from the tumor-inducing plasmid into plant genomes has also been reported during Agrobacterium tumefaciens–mediated transformation. We demonstrate that large (up to ∼18 kb) gene-bearing fragments of Agrobacterium chromosomal DNA (AchrDNA) can be integrated into Arabidopsis thaliana genomic DNA during transformation. One in every 250 transgenic plants may carry AchrDNA fragments. This has implications for horizontal gene transfer and indicates a need for greater scrutiny of transgenic plants for undesired bacterial DNA.

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T-DNA–mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants (1)

Similar content being viewed by others

T-DNA–mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants (3)

Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana

Article 17 October 2022

T-DNA–mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants (4)

Distinct mechanisms for genomic attachment of the 5′ and 3′ ends of Agrobacterium T-DNA in plants

Article 09 May 2022

References

  1. Gelvin, S. Microbiol. Mol. Biol. Rev. 67, 16–37 (2003).

    Article CAS Google Scholar

  2. Lacroix, B. et al. Trends Genet. 22, 29–37 (2006).

    Article CAS Google Scholar

  3. Smith, N. Trends Plant Sci. 3, 85 (1998).

    Article Google Scholar

  4. Wood, D.W. et al. Science 294, 2317–2323 (2001).

    Article CAS Google Scholar

  5. Goodner, B. et al. Science 294, 2323–2328 (2001).

    Article CAS Google Scholar

  6. Samson, F. et al. Nucleic Acids Res. 30, 94–97 (2002).

    Article CAS Google Scholar

  7. Sessions, A. et al. Plant Cell 14, 2985–2994 (2002).

    Article CAS Google Scholar

  8. Rosso, M.G. et al. Plant Mol. Biol. 53, 247–259 (2003).

    Article CAS Google Scholar

  9. Alonso, J.M. et al. Science 301, 653–657 (2003).

    Article Google Scholar

  10. Porter, R.D. Conjugation. in Modern Microbial Genetics 2nd edn. (eds. Streips, U.N. & Yasbin, R.E.) 463–506 (Wiley-Liss, New York, 2002).

    Chapter Google Scholar

  11. Timmerman, B. et al. J. Mol. Biol. 203, 373–384 (1988).

    Article CAS Google Scholar

  12. Mahillon, J. & Chandler, M. Microbiol. Mol. Biol. Rev. 62, 725–774 (1998).

    CAS PubMed PubMed Central Google Scholar

  13. Miranda, A. et al. J. Bacteriol. 174, 2288–2297 (1992).

    Article CAS Google Scholar

  14. Van Haaren, M.J. et al. Plant Mol. Biol. 13, 523–531 (1989).

    Article CAS Google Scholar

  15. Rommens, C.M. et al. Plant Physiol. 139, 1338–1349 (2005).

    Article CAS Google Scholar

  16. White, F.F. et al. Nature 301, 348–350 (1983).

    Article CAS Google Scholar

  17. Ülker, B. et al. Transgenic Res. 11, 311–313 (2002).

    Article Google Scholar

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Acknowledgements

We thank the students Pino Cagna and Alexander Boes for initial inverse PCR experiments leading to the discovery of the Agrobacterium chromosomal DNA in the transgenic locus named PM (PaperMaker) in a WRKY40 promoter::GUS line generated by Hikaru Seki, Prisca Viehoever for technical assistance and Csaba Koncz and Steven Spiker for suggestions and critical reading of the manuscript. This work was supported in part by the Max-Planck Society, the EU-funded REGIA project and the BMBF-funded GABI-Kat project.

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  1. Bekir Ülker,Yong Li&Mario G Rosso

    Present address: Present addresses: School of Biological and Biomedical Sciences, Durham University, Science Site, South Road, Durham DH1 3LE, UK (B.U.); University of Freiburg, FRISYS Core Facility Data Management, Habsburgerstrasse 49, D-79104 Freiburg, Germany (Y.L.); Leibniz-Institute of Plant Genetics and Crop Plant Research, Department of Molecular Genetics, Corrensstrasse 3, D-06466 Gatersleben, Germany (M.G.R.).,

Authors and Affiliations

  1. Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, Cologne, D-50829, Germany

    Bekir Ülker,Elke Logemann&Imre E Somssich

  2. Institute for Genome Research and Systems Biology, Bielefeld University, Universitaetsstrasse 25, Bielefeld, D-33594, Germany

    Yong Li,Mario G Rosso&Bernd Weisshaar

  3. Department of Molecular Plant Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, Cologne, D-50829, Germany

    Bernd Weisshaar

Authors

  1. Bekir Ülker

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Correspondence to Bekir Ülker or Bernd Weisshaar.

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Figures 1–5, Table 1, Methods, Data (PDF 647 kb)

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Ülker, B., Li, Y., Rosso, M. et al. T-DNA–mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants. Nat Biotechnol 26, 1015–1017 (2008). https://doi.org/10.1038/nbt.1491

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T-DNA–mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants (2024)
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